Ca2+ mobilization controls granule movement of insulin granules
نویسندگان
چکیده
منابع مشابه
Regulation of two insulin granule populations within the reserve pool by distinct calcium sources.
Insulin granule trafficking is a key step of glucose-stimulated insulin secretion from pancreatic beta cells. Using quantitative live cell imaging, we examined insulin granule movements within the reserve pool upon secretory stimulation in betaTC3 cells. For this study, we developed a custom image analysis program that permitted automatic tracking of the individual motions of over 20,000 granul...
متن کاملCa2+-dependent binding of cytosolic components to insulin-secretory granules results in Ca2+-dependent protein phosphorylation.
1. Incubation of a rat islet cell tumour homogenate with [gamma-32P]ATP resulted in the Ca2+-dependent phosphorylation of 100000-, 57000-, 29000-, 26000- and 14000-Mr proteins. The Ca2+ concentration required was in the low-microM range. 2. Isolated insulin granules did not exhibit Ca2+-dependent protein phosphorylation, whereas a soluble protein fraction showed the Ca2+-dependent phosphorylati...
متن کاملResource Mobilization Theory: Political Movement in Egypt
This paper discusses the application of the resource mobilization theory (RMT) to clarify the activities of certain key socio-political groups and movements in Egypt up till 2011. It contends that the political movements in the country have utilized information and communication technologies (ICTs) and social networks sites (SNSs) as a tool for mobilizing people and coordinating anti-regime act...
متن کاملPriming of insulin granules
Priming of secretory vesicles prior to Ca2+-induced exocytosis involves pairing of SNARE proteins on the vesicles and in the target membrane and biochemical loading by ATP-hydrolysis. Different priming enzymes have been proposed such as Nethylmaleimide Sensitive Factor (NSF) (Hanson et al., 1997; Sutton et al., 1998) and phosphatidylinositol-4-phosphate-5kinase (PtdInsP5K) (Hay et al., 1995), b...
متن کاملImaging exocytosis of single insulin secretory granules with evanescent wave microscopy: distinct behavior of granule motion in biphasic insulin release.
To study insulin exocytosis by monitoring the single insulin secretory granule motion, evanescent wave microscopy was used to quantitatively analyze the final stage of insulin exocytosis with biphasic release. Green fluorescent protein-tagged insulin transfected in MIN6 beta cells was packed in insulin secretory granules, which appeared to preferentially dock to the plasma membrane. Upon fusion...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1997
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)44918-4